PYD and CARD Domain Containing (PYCARD) antibody
| Antigen |
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| Synonyms |
ASC, TMS, TMS1, CARD5, TMS-1, MGC10332, Asc, PYCARD |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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Western Blotting (WB) (35), Immunofluorescence (IF) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), ELISA (15), Immunocytochemistry (ICC) (7), Immunoprecipitation (IP) (6), Immunohistochemistry (IHC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN183475 |
| Quantity |
0.1 mg |
| Price |
251.90 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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CARD5 / PYCARD / ASC
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Gene ID
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PYCARD
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UniProt
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Q9EPB4
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Immunogen
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The immunogen for anti-PYCARD antibody: synthetic peptide directed towards the middle region of mouse PYCARD
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Sequence
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TVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTG HFVDQHRQAL
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Cross-Reactivity
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Mouse (Murine), Rat (Rattus)
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Format
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Lyophilized
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Reconstitution
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Add 100 µl of distilled water. Final anti-PYCARD antibody concentration is 1 mg/ml.
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Description
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Pycard is a cytosolic soluble protein that forms insoluble aggregates and enhances etoposide-induced apoptosis.
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Characteristics
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Antigen Size: 193 amino acids
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Molecular Weight
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21kDa
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Application Notes
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WB Suggested Anti-PYCARD Antibody Titration: 2.5ug/ml
ELISA Titer: 1:62500
Positive Control: Human Thymus.
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Purification
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Protein A purified
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Buffer
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PBS
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Storage (Long Term)
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For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Storage (Short Term)
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Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
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Restrictions
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For Research Use only
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Product
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Mariathasan, Newton, Monack et al.: "Differential activation of the inflammasome by caspase-1 adaptors ASC and Ipaf." in: Nature, Vol. 430, Issue 6996, pp. 213-8, 2004 (PubMed).
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Alternatives for antigen "PYD and CARD Domain Containing (PYCARD)", type "Antibodies"
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Hosts
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Rabbit (47), Goat (6), Mouse (1)
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Reactivities
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Human (51), Mouse (Murine) (24), Rat (Rattus) (20), Cow (Bovine) (18), Dog (Canine) (18), Horse (Equine) (18), Pig (Porcine) (18)
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Applications
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Western Blotting (WB) (35), Immunofluorescence (IF) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), ELISA (15), Immunocytochemistry (ICC) (7), Immunoprecipitation (IP) (6), Immunohistochemistry (IHC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (6), AA 182-195 (3), N-Term (3), AA 31-46 (1), AA 83-102 (1), Internal Region (1)
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Alternatives 
2013 BioMed Central Research Awards
