PYCARD antibody (Internal Region)

Catalog No. ABIN1590015

The Goat Polyclonal anti-PYCARD antibody (ABIN1590015) specifically detects PYCARD in ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PYCARD antibody (Internal Region) (ABIN1590015)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-PYCARD Antibody

Binding Specificity: Internal Region

Purpose: Pycard (rodent)

Sequence: DLTDKLVSYY LES

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLTDKLVSYYLES, from the internal region of the protein sequence according to NP_075747.3.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3, KNRK and HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: Pycard

Background: Pycard, PYD and CARD domain containing, 9130417A21Rik, Asc, CARD5, TMS-1, TNS1, masc, PYD and CARD domain-containing protein, apoptosis-associated speck-like protein containing a CARD

Gene ID: 29108, 66824, 282817

NCBI Accession: NP_075747

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome