ISL LIM Homeobox 2 (ISL2) (C-Term) antibody

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Antigen
Epitope
C-Term
(22), (19), (4), (3), (3), (3), (2), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(69), (28), (26), (4), (3), (2), (1), (1), (1)
Host
Rabbit
(57), (13)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(55), (30), (11), (9), (4), (4), (3), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Synthetic peptide directed towards the C terminal of mouse ISL2
Sequence VIRVWFQNKR CKDKKKSILM KQLQQQQHSD KASFQGLTGT PLVAGSPIGH
Predicted Reactivity Mouse : 100 %, Rat : 90 %, Chicken : 80 %, Guinea pig : 80 %, Human : 80 %, Rabbit : 80 %, Bovine : 70 %, Horse : 70 %
Characteristics This is a rabbit polyclonal antibody against ISL2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name ISL2 (ISL2 Antibody Abstract)
Background Isl2 specifies RGC laterality by repressing an ipsilateral pathfinding program unique to VTC RGCs and involving Zic2 and EphB1. This genetic hierarchy controls binocular vision.
Molecular Weight 39 kDa
Gene ID 104360
NCBI Accession NP_081673, NM_027397
UniProt Q9CXV0
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 359 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-ISL LIM Homeobox 2 (ISL2) (C-Term) antibody (ABIN183487) WB Suggested Anti-ISL2 Antibody Titration: 5.0ug/ml
ELISA Titer: 1:3...
Product cited in: Svärd, Heby-Henricson, Henricson et al.: "Genetic elimination of Suppressor of fused reveals an essential repressor function in the mammalian Hedgehog signaling pathway." in: Developmental cell, Vol. 10, Issue 2, pp. 187-97, 2006 (PubMed).