CHRNB2 antibody (Middle Region)

Catalog No. ABIN183541

This Rabbit Polyclonal antibody specifically detects CHRNB2 in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Pig, Guinea Pig and Saccharomyces cerevisiae.

Quick Overview for CHRNB2 antibody (Middle Region) (ABIN183541)

Target: CHRNB2 (Cholinergic Receptor, Nicotinic, beta 2 (Neuronal) (CHRNB2))

Reactivity: Human, Rat, Mouse, Dog, Cow, Pig, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNB2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHRNB2 Antibody

Binding Specificity: Middle Region

Purpose: CHRNB2 Antibody - middle region

Sequence: KIEVKHFPFD QQNCTMKFRS WTYDRTEIDL VLKSEVASLD DFTPSGEWDI

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rat: 93%, Yeast: 77%

Characteristics: This is a rabbit polyclonal antibody against CHRNB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CHRNB2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8096965

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHRNB2

Target: CHRNB2 (Cholinergic Receptor, Nicotinic, beta 2 (Neuronal) (CHRNB2))

Alternative Name: CHRNB2

Background:

Targeted Subunit: beta-2

Background Information: Mutations in nAChRs are found in a rare form of nocturnal frontal lobe epilepsy . Previously, some nAChR mutations have been described that are associated with additional neurological features such as psychiatric disorders or cognitive defects. A new CHRNB2 mutation located in transmembrane region 3 (M3), outside the known ADNFLE mutation cluster. The CHRNB2 mutation I312M, which occurred de novo in twins, markedly increases the receptor's sensitivity to acetylcholine. Phenotypically, the mutation is associated not only with typical ADNFLE, but also with distinct deficits in memory. The cognitive problems are most obvious in tasks requiring the organization and storage of verbal information.

Gene Name: Cholinergic receptor, nicotinic, beta 2 (neuronal)

Alternative Symbols: EFNL3, nAChRB2

Protein Name: Neuronal acetylcholine receptor subunit beta-2

Molecular Weight: 57kDa

Gene ID: 1141

NCBI Accession: NP_000739

UniProt: P17787

Pathways: Sensory Perception of Sound, Feeding Behaviour