MECOM antibody (N-Term)

Catalog No. ABIN183666

The Rabbit Polyclonal anti-MECOM antibody (ABIN183666) specifically detects MECOM in WB. The antibody is reactive with Human, Mouse and Zebrafish (Danio rerio) samples.

Quick Overview for MECOM antibody (N-Term) (ABIN183666)

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECOM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MECOM Antibody

Binding Specificity: N-Term

Purpose: MDS1 Antibody - N-terminal region

Sequence: MRSKGRARKL ATNNECVYGN YPEIPLEEMP DADGVASTPS LNIQEPCSPA

Predicted Reactivity: Human: 100%, Mouse: 93%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against MDS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MDS1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8104980

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MECOM

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Alternative Name: MDS1

Background:

Background Information: MDS1 is located at 3q26 170-400 kb upstream (telomeric) of EVI1 in the chromosomal region in which some of the breakpoints 5' of EVI1 have been mapped. MDS1 has been identified as a single gene as well as a previously unreported exon(s) of EVI1. MDS1 exists in normal tissues both as a unique transcript and as a normal fusion transcript with EVI1, with an additional 188 codons at the 5' end of the previously reported EVI1 open reading frame. In cells with translocation t (3,21), additional fusion transcripts are AML1-MDS1 and AML1-MDS1-EVI1. EVI1 and MDS1 are involved in leukemia associated with chromosomal translocation breakpoints in the region between these genes.

Gene Name: Myelodysplasia syndrome 1

Alternative Symbols: PRDM3, MDS1-EVI1

Protein Name: MDS1 and EVI1 complex locus protein MDS1

Molecular Weight: 19kDa

Gene ID: 4197

NCBI Accession: NP_004982

UniProt: Q13465