MECOM antibody (C-Term)

Catalog No. ABIN2777403

The Rabbit Polyclonal anti-MECOM antibody is suitable to detect MECOM in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Rabbit. It has been validated for WB and IHC.

Quick Overview for MECOM antibody (C-Term) (ABIN2777403)

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECOM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MECOM Antibody

Binding Specificity: C-Term

Purpose: EVI1 Antibody - C-terminal region

Sequence: HFTDSLKMRK MEDNQYSEAE LSSFSTSHVP EELKQPLHRK SKSQAYAMML

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EVI1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EVI1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31659-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MECOM

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Alternative Name: EVI1

Background:

Background Information: EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity.

Gene Name: MDS1 and EVI1 complex locus

Alternative Symbols: EVI1, MDS1, KMT8E, PRDM3, RUSAT2, MDS1-EVI1, AML1-EVI-1

Protein Name: MDS1 and EVI1 complex locus protein EVI1

Molecular Weight: 118kDa

Gene ID: 2122

NCBI Accession: NP_005232

UniProt: Q03112