Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) antibody

Antigen: Hypoxia Inducible Factor 3, alpha Subunit (HIF3A)

Synonyms: Ipas, MOP7, NEPAS, bHLHe17, IPAS, PASD7, HIF-3A, HIF-3A2, HIF-3A4, Mop7, HIF3A, MGC63965, zgc:63965

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN183862

Additional Information

Alternative name: HIF3A / HIF3 alpha

Gene ID: HIF3A

Immunogen: The immunogen for anti-HIF3A antibody: synthetic peptide directed towards the C terminal of human HIF3A

Sequence: PGSLQDPSTPLLNLNEPLGLGPSLLSPYSDEDTTQPGGPF QPRAGSAQAD

Cross-Reactivity: Human

Format: Lyophilized

Reconstitution: Add 100 µl of distilled water. Final anti-HIF3A antibody concentration is 1 mg/ml.

Description: HIF3A is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression.The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene.

Characteristics: Antigen Size: 667 amino acids

Molecular Weight: 72kDa

Application Details

Application Notes: WB Suggested Anti-HIF3A Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate.

Purification: Protein A purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-HIF3A Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate

anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) antibody (Image 2)

Publications

Product: Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Lazrek, Goffard, Schanen et al.: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006 (PubMed).