Fas (TNFRSF6)-Associated Via Death Domain (FADD) antibody
| Antigen |
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| Synonyms |
GIG3, MORT1, MGC8528, Mort1/FADD |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Sepharose (5), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
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| Catalog no. |
ABIN184143 |
| Quantity |
50 µg (Variants) |
| Price |
317.90 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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FADD
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Gene ID
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FADD
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UniProt
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Q13158
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Immunogen
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The immunogen for anti-FADD antibody: synthetic peptide directed towards the C terminal of human FADD
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Sequence
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AHLVGALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSW NSDASTSEAS
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Cross-Reactivity
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Human
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Format
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Lyophilized
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Reconstitution
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Add 50 µl of distilled water. Final anti-FADD antibody concentration is 1 mg/ml.
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Description
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The protein encoded by FADD is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade.
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Characteristics
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Antigen Size: 208 amino acids
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Molecular Weight
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23kDa
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Application Notes
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WB Suggested Anti-FADD Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Thymus.
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Purification
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Affinity Purified
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Buffer
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PBS
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Storage (Long Term)
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For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Storage (Short Term)
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Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
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Research Area
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Cancer, Apoptosis/Necrosis
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Restrictions
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For Research Use only
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Product
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Thomas, Henson, Reed et al.: "Direct binding of Fas-associated death domain (FADD) to the tumor necrosis factor-related apoptosis-inducing ligand receptor DR5 is regulated by the death effector domain of FADD." in: The Journal of biological chemistry, Vol. 279, Issue 31, pp. 32780-5, 2004 (PubMed).
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Alternatives for antigen "Fas (TNFRSF6)-Associated Via Death Domain (FADD)", type "Antibodies"
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Hosts
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Rabbit (66), Mouse (26)
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Reactivities
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Human (83), Mouse (Murine) (36), Rat (Rattus) (24), Pig (Porcine) (18), Dog (Canine) (2), Monkey (2), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1)
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Applications
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Western Blotting (WB) (58), ELISA (37), Immunofluorescence (IF) (35), Immunohistochemistry (IHC) (15), Immunoprecipitation (IP) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Sepharose (5), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Epitopes
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pSer194 (11), pSer191 (8), C-Term (3), AA 1-208 (2), AA 125-140 (1), Internal Region (1), N-Term (1), Ser194 (1), pSer71 (1), pThr567 (1)
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2013 BioMed Central Research Awards
