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E2F Transcription Factor 1 (E2F1) antibody

Antigen

E2F Transcription Factor 1 (E2F1)
Synonyms RBP3, E2F-1, RBAP1, RBBP3, KIAA4009, mKIAA4009, E2F1, xE2F
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Cow (Bovine), Human, Mouse (Murine), Dog (Canine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
1 reference available
Catalog no. ABIN184178
Quantity 50 µg  (Variants)
Price 317.90 $   Plus shipping costs $45.00
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anti-E2F Transcription Factor 1 (E2F1) antibody

Additional Information
Application Details Publications Images Alternatives Variants back to top

Alternative name E2F1
Gene ID E2F1
UniProt Q01094
Immunogen The immunogen for anti-E2F1 antibody: synthetic peptide directed towards the C terminal of human E2F1
Sequence REDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDC DFGDLTPLDF
Cross-Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine)
Format Lyophilized
Reconstitution Add 50 µl of distilled water. Final anti-E2F1 antibody concentration is 1 mg/ml.
Description The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Characteristics Antigen Size: 437 amino acids
Molecular Weight 47kDa

Application Details
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Application Notes WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Daudi cell lysate.
Purification Affinity Purified
Buffer PBS
Storage (Long Term) For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Storage (Short Term) Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
Research Area Cancer, Transcription Factors
Restrictions For Research Use only

Publications
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Product Unoki, Nishidate, Nakamura: "ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain." in: Oncogene, Vol. 23, Issue 46, pp. 7601-10, 2004 (PubMed).

Alternatives
Application Details Publications Images Variants back to top

Alternatives for antigen "E2F Transcription Factor 1 (E2F1)", type "Antibodies"
Hosts Rabbit (132), Mouse (39), Goat (1), Sheep (1)
Reactivities Human (168), Mouse (Murine) (73), Rat (Rattus) (64), Horse (Equine) (23), Cow (Bovine) (20), Dog (Canine) (20), Guinea Pig (18), Pig (Porcine) (18), Chicken (2), Cat (Feline) (1), Chimpanzee (1), Fruit Fly (Drosophila melanogaster) (1), Monkey (1), Xenopus laevis (1)
Applications Western Blotting (WB) (96), Immunofluorescence (IF) (78), ELISA (57), Immunoprecipitation (IP) (37), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Immunohistochemistry (IHC) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Gel Shift (GS) (8), Immunocytochemistry (ICC) (7), Flow Cytometry (FACS) (5), Dot Blot (Dot) (4), Immunoelectron Microscopy (IEM) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Biotin (7), Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), FITC (4), PE,Cy5.5 (4), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), HRP (3), PE (3), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2), Un-conjugated (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer337 (14), Ser337 (13), Thr433 (12), pSer364 (10), pThr433 (7), AA 162-476 (6), pSer332 (6), AA 244-264 (5), Internal Region (4), N-Term (4), AA 223-322 (3), AA 332-390 (3), AA 223-332 (2), C-Term (2), His357 (2), Ser332 (2), AA 310-329 (1), AA 360-369,pSer364 (1), AA 69-223 (1), Center (1), Middle Region (1), pHis357 (1), pSer1073 (1), pTyr892 (1)