GABPA antibody (C-Term)

Catalog No. ABIN184314

The Rabbit Polyclonal anti-GABPA antibody (ABIN184314) specifically detects GABPA in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Dog, Cow, Horse and Zebrafish (Danio rerio) samples.

Quick Overview for GABPA antibody (C-Term) (ABIN184314)

Target: GABPA (GA Binding Protein Transcription Factor, alpha Subunit 60kDa (GABPA))

Reactivity: Human, Rat, Mouse, Dog, Cow, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GABPA Antibody

Binding Specificity: C-Term

Sequence: KWGQRKNKPT MNYEKLSRAL RYYYDGDMIC KVQGKRFVYK FVCDLKTLIG

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against GABPA. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GABPA

Application Details

Application Notes: WB Suggested Anti-GABPA Antibody Titration: 0.5-1.0 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 454 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GABPA

Target: GABPA (GA Binding Protein Transcription Factor, alpha Subunit 60kDa (GABPA))

Alternative Name: GABPA

Background: GA Binding Protein . chain (GABP-. subunit, GABPA, nuclear respiratory factor-2 subunit . transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.
Alias Symbols: NFT2, NRF2, NRF2A, E4TF1A, E4TF1-60, RCH04A07
Protein Interaction Partner: UBC, NCOA7, KCTD12, TSEN34, DIABLO, CUTC, RAB3GAP2, LIMCH1, RAB3GAP1, GNE, KPNA1, YY1, GABPB1, PINX1, APP, KEAP1, SUMO2, HDAC1, EP300, SP3, CREBBP, HCFC1, SP1, ATF1, MED1,
Protein Size: 454

Molecular Weight: 51 kDa

Gene ID: 2551

NCBI Accession: NM_002040, NP_002031

UniProt: Q06546

Pathways: Myometrial Relaxation and Contraction