DDB1 antibody (C-Term)

Catalog No. ABIN184570

This Goat Polyclonal antibody specifically detects DDB1 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for DDB1 antibody (C-Term) (ABIN184570)

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DDB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-DDB1 Antibody

Binding Specificity: C-Term

Purpose: DDB1

Sequence: DLIKVVEELT RIH

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLIKVVEELTRIH, from the C Terminus of the protein sequence according to NP_001914.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 140 kDa band observed in lysates of cell line HeLa, HepG2 and Jurkat (calculated MW of 126.9 kDa according to Human NP_001914.3). Recommended concentration: 1-2 μg/mL. Approx 140 kDa band observed in lysates of cell line NIH3T3 and app

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-DDB1 antibody (ABIN184570)

Li, Robert, van Breugel, Strubin, Zheng: "A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery." in: Nature structural & molecular biology, Vol. 17, Issue 1, pp. 105-11, (2010) (PubMed).

Target Details for DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Alternative Name: DDB1

Background: DDB1, damage-specific DNA binding protein 1, 127 kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD), XPE

Gene ID: 1642, 64470

NCBI Accession: NP_001914

Pathways: DNA Damage Repair