IL2-Inducible T-Cell Kinase (ITK) antibody

Antigen: IL2-Inducible T-Cell Kinase (ITK)

Synonyms: EMT, LYK, PSCTK2, MGC126257, MGC126258, Emt, Tsk, Tcsk

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184605

Additional Information

Alternative name: ITK

Gene ID: 3702, 16428 (mouse)

Immunogen: C-RLLRQLAEIAESGL

Format: Affinity-purified

Description: All names and symbols: ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK. Official Symbol: ITK. RefSeq number(s): NP_005537. Gene ontology terms: non-membrane spanning protein tyrosine kinase activity, ATP binding, protein amino acid phosphorylation, cellular defense response, intracellular signaling cascade, transferase activity

Specificity: Human, Mouse

Application Details

Application Notes: Western blot: Approx 75 kDa bands seen in Jurkat Lysates. Recommended for use at 0.5-2 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Tyrosine Kinases

Restrictions: For Research Use only

Images

ABIN184605 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Tanaka, Asao, Ohtani et al.: "A novel human tyrosine kinase gene inducible in T cells by interleukin 2." in: FEBS letters, Vol. 324, Issue 1, pp. 1-5, 1993 (PubMed).