C-Src tyrosine Kinase (CSK) antibody

Antigen: C-Src tyrosine Kinase (CSK)

Synonyms: MGC117393, AW212630, MGC112926

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184606

Additional Information

Alternative name: CSK

Gene ID: 1445, 12988 (mouse)

Immunogen: C-EQLEHIKTHELHL

Format: Affinity-purified

Description: All names and symbols: CSK, c-src tyrosine kinase. Official Symbol: CSK. RefSeq number(s): NP_004374.1. Gene ontology terms: protein C-terminus binding activity, protein tyrosine kinase activity, protein amino acid phosphorylation, regulation of cell cycle, cytoplasm

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 50kDa band observed in Jurkat lysate (predicted MW of 50.7kDa according to NP_004374.1). Recommended for use at 0.5-2µg/ml. Immunohistochemistry: In paraffin embedded human tonsil, strong staining of germinal centre cells and less intense labelling in the interfollicular area. Recommended concentration 0.1-0.3ug/ml

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Tyrosine Kinases

Restrictions: For Research Use only

Images

ABIN184606 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN184606 (0.1µg/ml) staining of paraffin embedded human tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining of germinal centres. B) High magnification view of germinal centre cells.

Publications

Publications: Holtrich, Braeuninger, Strebhardt et al.: "Two additional protein-tyrosine kinases expressed in human lung: fourth member of the fibroblast growth factor receptor family and an intracellular protein-tyrosine kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 23, pp. 10411-5, 1992 (PubMed).