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SMAD, Mothers Against DPP Homolog 4 (SMAD4) antibody
| Antigen | SMAD, Mothers Against DPP Homolog 4 (SMAD4) |
| Synonyms | JIP, DPC4, MADH4, SMAD4 |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Mouse (Murine) |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN184619 |
| Quantity | 100 ug (Variants) |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | SMAD4 / MADH4 |
| Gene ID | 4089, 50554 (rat) |
| Immunogen | C-HTMPIADPQPLD |
| Format | Affinity-purified |
| Description | All names and symbols: MADH4, SMAD4, JIP, DPC4, MAD, mothers against decapentaplegic homolog 4 (Drosophila), Mothers against decapentaplegic, Drosophila, homolog of, 4, MAD (mothers against decapentaplegic, Drosophila) homolog 4. Official Symbol: SMAD4. RefSeq number(s): NP_005350.1. Gene ontology terms: transcription cofactor activity, transcription factor activity, SMAD protein heteromerization, regulation of transcription, DNA-dependent, cytoplasm, nucleus |
| Specificity | Human, Mouse, Rat |
Application Details
| Application Notes | Western blot: Approx 60-65kDa band seen in A431 cell lysate. Recommended for use at 0.5-2µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN184619 staining (0.5µg/ml) of NIH-3T3 cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
Publications
| Publications |
Kim, Fan, Papadimitrakopoulou et al.: "DPC4, a candidate tumor suppressor gene, is altered infrequently in head and neck squamous cell carcinoma." in: Cancer research, Vol. 56, Issue 11, pp. 2519-21, 1996 (PubMed).
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Alternatives
Alternatives for antigen "SMAD, Mothers Against DPP Homolog 4 (SMAD4)", type "Antibodies"
| Hosts | Rabbit (34), Mouse (23), Goat (3) |
| Reactivities | Human (58), Mouse (Murine) (27), Rat (Rattus) (27), Cow (Bovine) (3), Horse (Equine) (3), Rabbit (3), Dog (Canine) (2), Pig (Porcine) (2), Monkey (1), Xenopus laevis (1) |
| Applications | Western Blotting (WB) (38), Immunofluorescence (IF) (26), Enzyme Immunoassay (EIA) (20), Flow Cytometry (FACS) (19), ELISA (9), Immunoprecipitation (IP) (6), Dot Blot (Dot) (3), Immunohistochemistry (IHC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | T277 (2) |
