FYN Oncogene Related To SRC, FGR, YES (FYN) antibody

Antigen: FYN Oncogene Related To SRC, FGR, YES (FYN)

Synonyms: FYN, fyn, MGC52878, MGC132140, zgc:109996, c-fyn, zgc:86720, SLK, SYN, MGC45350, AI448320, AW552119, MGC115870, PKC

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184620

Additional Information

Alternative name: FYN

Gene ID: 2534, 14360 (mouse), 25150 (rat)

Immunogen: VQCKDKEATKLTE

Format: Affinity-purified

Description: All names and symbols: FYN, SLK, SYN, MGC45350, FYN oncogene related to SRC, FGR, YES, src-like kinase, c-syn protooncogene, tyrosine kinase p59fyn(T), src/yes-related novel gene, OKT3-induced calcium influx regulator, proto-oncogene tyrosine-protein kinase fyn. Official Symbol: FYN. RefSeq number(s): NP_002028.1, NP_694592.1, NP_694593.1. Gene ontology terms: learning, feeding behavior, protein kinase cascade, protein phosphorylation, protein tyrosine kinase

Specificity: This antibody is expected to recognise all three reported isoforms (as represented by NP_002028.1, NP_694592.1, NP_694593.1). Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 55-60kDa band observed in Jurkat, A431 and NIH-3T3 lysates (predicted MW of 62kDa according to NP_002028.1). Recommended for use at 0.2-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184620 staining 0.2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Semba, Nishizawa, Miyajima et al.: "yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 15, pp. 5459-63, 1986 (PubMed).