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Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP) antibody

Antigen

Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP)

Synonyms Mal, wyatt, FLJ42305, mal, tirap, MGC83732, TIRAP
Clonality Polyclonal
Host
Reactivity
Alternatives

Human

Application
Alternatives ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN184633
Quantity 100µg  (100µg specific antibody in 200µl)  (Variants)
Price 501.80 $   Plus shipping costs $35.00
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Additional Information

Alternative name TIRAP / Mal (Isoform b)
Gene ID 114609
Immunogen Peptide with sequence C-EGEGERDSATVSDL, from the C Terminus of the protein sequence according to NP_683708.1
Format Affinity-purified
Description All names and symbols: TIRAP, Mal, toll-interleukin 1 receptor (TIR) domain-containing adapter protein, adapter protein wyatt, MyD88 adapter-like protein, FLJ42305, wyatt, OTTHUMP00000179130, Toll-interleukin 1 receptor domain-containing adaptor protein, Toll-like receptor adaptor protein, adapter protein wyatt. Official Symbol: TIRAP. RefSeq number(s): NP_683708.1.
Specificity Expected from sequence similarity: Human

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 30kDa band observed in Human Liver lysates (calculated MW of 25.3kDa according to NP_683708.1). Recommended concentration: 1-3µg/ml.
Concentration 100µg specific antibody in 200µl
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Publications Andreakos, Sacre, Smith et al.: "Distinct pathways of LPS-induced NF-kappa B activation and cytokine production in human myeloid and nonmyeloid cells defined by selective utilization of MyD88 and Mal/TIRAP." in: Blood, Vol. 103, Issue 6, pp. 2229-37, 2004 (PubMed).

Alternatives

Alternatives for antigen "Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP)", type "Antibodies"
Hosts Rabbit (52), Goat (9), Mouse (5), Rat (3)
Reactivities Human (56), Mouse (Murine) (27), Rat (Rattus) (19), Cow (Bovine) (5), Dog (Canine) (5), Chicken (4), Horse (Equine) (3), Rabbit (2), Zebrafish (Brachydanio rerio) (2), Pig (Porcine) (1)
Applications Western Blotting (WB) (47), Flow Cytometry (FACS) (16), Immunofluorescence (IF) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), ELISA (10), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (IHC) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunoprecipitation (IP) (3), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes C-Term (11), Center (1), N-Term (1), internal (1), pTyr86 (1)