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Antibody
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anti-Activating Transcription Factor 2 ATF2
Activating Transcription Factor 2 (ATF2) antibody
| Antigen |
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| Synonyms |
HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (104), Immunohistochemistry (IHC) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (32), Enzyme Immunoassay (EIA) (30), ELISA (23), Flow Cytometry (FACS) (14), Dot Blot (Dot) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
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| Catalog no. |
ABIN184639 |
| Quantity |
100 ug (Variants) |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business days |
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Alternative name
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ATF2
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Gene ID
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1386, 81647 (rat)
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Immunogen
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C-IVMAPSSQSQPSGS
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Format
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Ig-fraction
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Description
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All names and symbols: ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558. Official Symbol: ATF2. RefSeq number(s): NP_001871.2. Gene ontology terms: RNA polymerase II transcription factor activity, transcription co-activator activity
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Specificity
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Human, Rat
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approx 55kDa and 30kDa in lysates of Human Placenta and of cell line HeLa after 0.5µg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Bailey et al , J Clin Endocrinol Metab. 2002 Apr,87(4):1717-28., PMID: 11932306). This protein has a calculated MW of 54.5kDa according to NP_001871.2. Recommended concentration: 0.5-1.5µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Immunology, Innate Immunity, Transcription Factors
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Restrictions
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For Research Use only
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Publications
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Monzen, Hiroi, Kudoh et al.: "Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation." in: The Journal of cell biology, Vol. 153, Issue 4, pp. 687-98, 2001 (PubMed).
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Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
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Hosts
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Rabbit (143), Mouse (16), Goat (3), Sheep (3)
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Reactivities
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Human (162), Mouse (Murine) (112), Rat (Rattus) (99), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1)
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Applications
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Western Blotting (WB) (104), Immunohistochemistry (IHC) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (32), Enzyme Immunoassay (EIA) (30), ELISA (23), Flow Cytometry (FACS) (14), Dot Blot (Dot) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pThr69/51 (6), Ab-112 or 94 (5), Ab-62 or 44 (5), Ab-69 or 51 (5), Ab-71 or 53 (5), Ab-73 or 55 (5), pSer112 or 94 (5), pSer62 or 44 (5), pSer62/44 (5), pThr69 or 51 (5), pThr71 or 53 (5), pThr73 or 55 (5), pSer112/94 (4), pThr71/53 (4), pThr73/55 (3), S44 (2), S94 (2), PSer340/322 (1), S322 (1), pSer44 (1), pSer472 (1), pSer94 (1), pThr51 (1), pThr69/51 + pThr71/53 (1), pThr71 (1), pThr79/51 (1)
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Alternatives 
