PPP2R5D antibody (C-Term)

Catalog No. ABIN184648

This Goat Polyclonal antibody specifically detects PPP2R5D in WB, ELISA, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PPP2R5D antibody (C-Term) (ABIN184648)

Target: PPP2R5D (Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R5D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PPP2R5D Antibody

Binding Specificity: C-Term

Purpose: PPP2R5D

Sequence: KRAEEFLTAS QEAL

Specificity: This antibody is expected to recognise reported isoforms (NP_006236.1 (iso 1), NP_851307.1, (iso 2) NP_851308.1) (iso 3) and NP_001257405.1 (iso 4).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KRAEEFLTASQEAL, from the C Terminus of the protein sequence according to NP_006236.1, NP_851307.1, NP_851308.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in lysates of cell lines A431, HepG2 and Jurkat, and in Human Cerebellum lysates, approx. 65-70 kDa in lysates of cell line NIH3T3, and approx.75 kDa in Rat Brain lysates, (calculated MW of 70 kDa according to Huma

Peptide ELISA: antibody detection limit dilution 1:20000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP2R5D

Target: PPP2R5D (Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D))

Alternative Name: PPP2R5D

Background: PPP2R5D, MGC2134, MGC8949, protein phosphatase 2, regulatory subunit B (B56), delta isoform, PP2A, B subunit, B' delta isoform, PP2A, B subunit, R5 delta isoform, PP2A, B subunit, B56 delta isoform, PP2A, B subunit, PR61 delta isoform, Serine/threonine pr

Gene ID: 5528

NCBI Accession: NP_006236, NP_851307, NP_851308

Pathways: PI3K-Akt Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades