Protein Phosphatase 2, Regulatory Subunit B', alpha (PPP2R5A) antibody

Antigen: Protein Phosphatase 2, Regulatory Subunit B', alpha (PPP2R5A)

Synonyms: B56A, PR61A, MGC131915, MGC38798, PR61alpha, PPP2R5A, MGC138101, si:dkey-18c8.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184649

Additional Information

Alternative name: PPP2R5A

Gene ID: 5525

Immunogen: C-AYNMHSILSNTSAE

Description: All names and symbols: PPP2R5A, protein phosphatase 2, regulatory subunit B (B56), alpha isoform, MGC131915 , PP2A, B subunit, B' alpha isoform, PP2A, B subunit, B56 alpha isoform, PP2A, B subunit, PR61 alpha isoform, PP2A, B subunit, R5 alpha isoform, serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alpha isoform. Official Symbol: PPP2R5A. RefSeq number(s): NP_006234.1. Gene ontology terms: protein phosphatase type 2A, regulator activity, cytoplasm

Specificity: Human, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1/32,000. Western Blot: Approx 55kDa band observed in human heart and skeletal muscle lysate (calculated MW of 56.2kDa according to NP_006234.1). Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN184649 staining (0.5µg/ml) of human muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

McCright, Virshup: "Identification of a new family of protein phosphatase 2A regulatory subunits." in: The Journal of biological chemistry, Vol. 270, Issue 44, pp. 26123-8, 1995 (PubMed).