Dual Specificity Phosphatase 16 (DUSP16) antibody

Antigen: Dual Specificity Phosphatase 16 (DUSP16)

Synonyms: DUSP16, MKP7, Mkpm, MKP-7, AW558566, D6Ertd213e, 3830417M17Rik, KIAA1700, MGC129701, MGC129702, dusp16, wu:fi04g09, mkp7, mkp-7

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN184650

Additional Information

Alternative name: DUSP16

Gene ID: 80824, 70686 (mouse)

Immunogen: AHEMIGTQIVTER-C

Description: All names and symbols: DUSP16, dual specificity phosphatase 16, MKP7, MKP-7, KIAA1700, MAPK phosphatase-7, MGC129701, MGC129702. Official Symbol: DUSP16. RefSeq number(s): NP_085143.1. Gene ontology terms: MAP kinase phosphatase activity, MAPK nucleus export, hydrolase activity, inactivation of MAPK, leptomycin B-sensitive MAPK phosphatase nucleus export, protein amino acid dephosphorylation,

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave no signal but low background in Human Brain and Jurkat lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry: In paraffin embedded Human Placenta shows individual cells in branch villi very close to the surrounding lacuna. Recommended concentration, 2-4µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN184650 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Tanoue, Yamamoto, Maeda et al.: "A Novel MAPK phosphatase MKP-7 acts preferentially on JNK/SAPK and p38 alpha and beta MAPKs." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 26629-39, 2001 (PubMed).