PPP2R5B antibody (C-Term)

Catalog No. ABIN184652

The Goat Polyclonal anti-PPP2R5B antibody is suitable to detect PPP2R5B in samples from Human. It has been validated for WB and ELISA.

Quick Overview for PPP2R5B antibody (C-Term) (ABIN184652)

Target: PPP2R5B (Protein Phosphatase 2, Regulatory Subunit B', beta (PPP2R5B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R5B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PPP2R5B Antibody

Binding Specificity: C-Term

Purpose: B56 beta isoform

Sequence: QRLTPQVAAS GGQS

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QRLTPQVAASGGQS, from the C Terminus of the protein sequence according to NP_006235.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 55 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 57.4 kDa according to NP_006235.1). Recommended concentration: 2-3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat B

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP2R5B

Target: PPP2R5B (Protein Phosphatase 2, Regulatory Subunit B', beta (PPP2R5B))

Alternative Name: PPP2R5B

Background: PPP2R5B, protein phosphatase 2, regulatory subunit B (B56), beta isoform, PP2A, B subunit, B' beta isoform, PP2A, B subunit, R5 beta isoform, PP2A, B subunit, B56 beta isoform, PP2A, B subunit, PR61 beta isoform, serine/threonine protein phosphatase 2A, 5

Gene ID: 5526

NCBI Accession: NP_006235

Pathways: PI3K-Akt Signaling, ER-Nucleus Signaling