B56 beta antibody

Antigen: B56 beta

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184652

Additional Information

Gene ID: 5526

Immunogen: C-QRLTPQVAASGGQS

Description: All names and symbols: PPP2R5B, protein phosphatase 2, regulatory subunit B (B56), beta isoform, PP2A, B subunit, B' beta isoform, PP2A, B subunit, R5 beta isoform, PP2A, B subunit, B56 beta isoform, PP2A, B subunit, PR61 beta isoform, serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, beta isoform. Official Symbol: PPP2R5B. RefSeq number(s): NP_006235.1. Gene ontology terms: protein phosphatase type 2A, intrinsic regulator activity, signal transduction, protein phosphatase type 2A complex, cytoplasm

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx. 55kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 57.4kDa according to NP_006235.1). Recommended concentration: 2-3µg/ml. Mouse has been succesfully tested with a previous batch.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184652 (2µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

McCright, Virshup: "Identification of a new family of protein phosphatase 2A regulatory subunits." in: The Journal of biological chemistry, Vol. 270, Issue 44, pp. 26123-8, 1995 (PubMed).