Cortactin (CTTN) antibody

Antigen: Cortactin (CTTN)

Synonyms: Ems1, 1110020L01Rik, EMS1, FLJ34459, DmelCG3637, CG3637, Cttnb, MGC93456, CTTN1, P85.25, Cttn, ems1, MGC80961, CTTN, cttn, MGC137923, DKFZp459H142

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184659

Additional Information

Alternative name: Cortactin / EMS1

Gene ID: 2017, 13043 (mouse)

Immunogen: WKASAGHAVSIA-C

Format: Affinity-purified

Description: All names and symbols: EMS1, CORTACTIN, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate), Cttn, oncogene EMS1. Official Symbol: CTTN. RefSeq number(s): NP_005222.2, NP_612632.1. Gene ontology terms: soluble fraction, peripheral plasma membrane protein, cytoskeleton

Specificity: This antibody is expected to recognize both reported isoforms (NP_005222.2, NP_612632.1). Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:xx,000. Western Blot: Approx. 80kDa band observed in Mouse and Rat Brain lysates (calculated MW of 61.6kDa according to Human NP_005222.2 and of 61.2kDa according to Mouse NP_031829.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paradis et al, J Cell Sci. 2008 Jun 15,121(Pt 12):1965-72, PMID 18495841). Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184659 (0.01µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Schuuring, Verhoeven, Mooi et al.: "Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas." in: Oncogene, Vol. 7, Issue 2, pp. 355-61, 1992 (PubMed).