PYD and CARD Domain Containing (PYCARD) antibody

Antigen: PYD and CARD Domain Containing (PYCARD)

Synonyms: ASC, TMS, TMS1, CARD5, TMS-1, MGC10332, Asc, PYCARD

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184674

Additional Information

Alternative name: ASC / TMS1

Gene ID: 29108

Immunogen: C-RESQSYLVEDLERS

Format: Affinity-purified

Description: All names and symbols: ASC, TMS1, PYCARD, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1. Official Symbol: PYCARD. RefSeq number(s): NP_037390.2, NP_660183.1, NP_660184.1. Gene ontology terms: caspase activation, caspase activator activity, cytoplasm, induction of apoptosis, negative regulation of cell cycle, protein binding, regulation of apoptosis, signal transduction,

Specificity: This antibody is expected to recognise all three reported Human isoforms according to NP_037390.2, NP_660183.1 and NP_660184.1. Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 22kDa band observed in Hela and Human Kidney lysates (predicted MW of 22kDa according to NP_037390.2). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184674 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing ASC (RC215592) and probed with ABIN184674 (mock transfection in first lane).

Publications

Publications: Ohtsuka, Ryu, Minamishima et al.: "ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway." in: Nature cell biology, Vol. 6, Issue 2, pp. 121-8, 2004 (PubMed).