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Antibodies
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anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) antibody
E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) antibody
| Antigen |
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| Synonyms |
Siah-1, hSIAH1, HUMSIAH, Siah-1a, FLJ08065, Siah1a, zgc:56026, id:ibd2635, SIAH, Siah1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunoprecipitation (IP)
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| Catalog no. |
ABIN184675 |
| Quantity |
100 µg (100µg/200µl) |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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SIAH1
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Gene ID
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6477, 20437 (mouse)
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Swiss-Prot
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NP_003022.3
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Immunogen
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Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3, NP_001006611.1.
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Cross-Reactivity
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Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
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Format
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Liquid
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Description
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Principal Names: sonic hedgehog homolog, seven in absentia homolog 1, FLJ08065, Siah-1a, HUMSIAH, hSIAH1, Siah-1, seven in absentia homolog 1 (Drosophila), SIAH1. Official Symbol: SIAH1
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Specificity
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This product is expected to recognize both reported isoforms (according to NP_003022.3, NP_001006611.1) Human, Mouse, Rat, Dog 282 aa
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Molecular Weight
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31.1kDa
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 28kDa band observed in Human Liver lysates (calculated MW of 31.1kDa according to NP_003022.3). Recommended concentration: 1-3µg/ml. Immunoprecipitation: This antibody has been successfully used in IP from CSM14.1 cells as described in the following paper: Liman et al, Molecules 2010, 15, 6678-6687.
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Concentration
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100µg/200µl
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Proteolysis / Ubiquitin
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Restrictions
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For Research Use only
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Publications
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Susini, Passer, Amzallag-Elbaz et al.: "Siah-1 binds and regulates the function of Numb." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 26, pp. 15067-72, 2001 (PubMed).
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Alternatives for antigen "E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1)", type "Antibodies"
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Hosts
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Rabbit (33), Mouse (4), Goat (3)
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Reactivities
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Human (33), Mouse (Murine) (20), Rat (Rattus) (20), Dog (Canine) (17), Chicken (16), Cow (Bovine) (16), Horse (Equine) (16), Pig (Porcine) (16), Rabbit (16), Guinea Pig (1)
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Applications
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Western Blotting (WB) (21), Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), ELISA (5), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (IHC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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