SIAH1 antibody (N-Term)

Catalog No. ABIN2778297

The Rabbit Polyclonal anti-SIAH1 antibody is suitable to detect SIAH1 in samples from Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse and Rabbit. It has been validated for WB and IHC.

Quick Overview for SIAH1 antibody (N-Term) (ABIN2778297)

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SIAH1 Antibody

Binding Specificity: N-Term

Purpose: SIAH1 Antibody - N-terminal region

Sequence: FTCLPAARTR KRKEMSRQTA TALPTGTSKC PPSQRVPALT GTTASNNDLA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SIAH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SIAH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099378

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SIAH1

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Alternative Name: SIAH1

Background:

Background Information: SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Gene Name: Siah E3 ubiquitin protein ligase 1

Alternative Symbols: SIAH1A

Protein Name: E3 ubiquitin-protein ligase SIAH1

Molecular Weight: 35kDa

Gene ID: 6477

NCBI Accession: NP_001006611

UniProt: Q8IUQ4