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Homolog (Drosophila) (NUMB) antibody
| Antigen | Homolog (Drosophila) (NUMB) |
| Synonyms | S171, mnb, m-numb, MGC188364, NUMB, z-numb, MGC123031, zgc:123031, MGC159835, s171, DKFZp469K1820, numb |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Mouse (Murine) |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
3 references available |
| Catalog no. | ABIN184679 |
| Quantity | 100 ug (Variants) |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | NUMB |
| Gene ID | 8650, 18222 (mouse) |
| Immunogen | C-PFSSDLQKTFEIEL |
| Format | Affinity-purified |
| Description | All names and symbols: NUMB, numb homolog (Drosophila), S171. Official Symbol: NUMB. RefSeq number(s): NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1. Gene ontology terms: integral to plasma membrane |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally. Human, Mouse, Dog 640 aa |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 80kDa band observed in Mouse Brain lysates (calculated MW of 70.8kDa according to NP_001005743.1). Recommended concentration: 0.03-0.1µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Research Area | Stem Cells |
| Restrictions | For Research Use only |
Images
| ABIN184679 staining (0.03µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
Publications
| Publications |
Zhong, Feder, Jiang et al.: "Asymmetric localization of a mammalian numb homolog during mouse cortical neurogenesis." in: Neuron, Vol. 17, Issue 1, pp. 43-53, 1996 (PubMed).
Sanada, Tsai: "G protein betagamma subunits and AGS3 control spindle orientation and asymmetric cell fate of cerebral cortical progenitors." in: Cell, Vol. 122, Issue 1, pp. 119-31, 2005 (PubMed). Holowacz, Zeng, Lassar: "Asymmetric localization of numb in the chick somite and the influence of myogenic signals." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 3, pp. 633-45, 2006 (PubMed). |
Alternatives
Alternatives for antigen "Homolog (Drosophila) (NUMB)", type "Antibodies"
| Hosts | Rabbit (31), Mouse (7), Goat (3), Sheep (1) |
| Reactivities | Human (40), Mouse (Murine) (21), Rat (Rattus) (18), Dog (Canine) (3), Chicken (2), Cow (Bovine) (2), Horse (Equine) (1) |
| Applications | Western Blotting (WB) (24), Flow Cytometry (FACS) (21), Immunofluorescence (IF) (19), ELISA (5), Enzyme Immunoassay (EIA) (4), ELISA (Detection) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | transcript variant 3 (2), N-Term G94 (1) |
