Calbindin 2 (CALB2) antibody

Antigen: Calbindin 2 (CALB2)

Synonyms: CAL2, CR, MGC105356, CALB2, MGC128318, P36, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, PAP-IV, Cal1h, AW215814, ANXA2, MGC53008, p36, anx2, lip2, lpc2, cal1h, lpc2d, pap-i, anx2l4, MGC76145, ANNEXIN, ANNEXIN A2, Annexin Arabidopsis 2, MXK3.27, MXK3_27, DKFZp469L2414

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184687

Additional Information

Alternative name: Calretinin

Gene ID: 302, 12306 (mouse), 56611 (rat)

Immunogen: STVHEILCKLSLEGD

Format: Affinity-purified

Description: All names and symbols: Calretinin, ANXA2, annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, annexin II, lipocortin II, Annexin II (lipocortin I), annexin II (lipocortin II), calpactin I, heavy polypeptide (p36), annexin II (lipocortin II calpactin I, heavy polypeptide), calpactin I heavy polypeptide, chromobindin 8, lipocortin II, placental anticoagulant protein IV. Official Symbol: ANXA2. RefSeq number(s): NP_001002858.1, NP_004030.1, NP_001002857.1. Gene ontology terms: phospholipase inhibitor activity, calcium-dependent phospholipid binding, calcium ion binding, skeletal development, soluble fraction, plasma membrane, anticoagulant activity

Specificity: This antibody is expected to recognise both reported isoforms. NP_004030.1 and NP_001002857.1 are varients of isoform 2. Human, Mouse, Rat, Cow, Pig

Synonyms: CAL2, CR, MGC105356, CALB2, MGC128318, P36, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, PAP-IV, Cal1h, AW215814, ANXA2, MGC53008, p36, anx2, lip2, lpc2, cal1h, lpc2d, pap-i, anx2l4, MGC76145, ANNEXIN, ANNEXIN A2, Annexin Arabidopsis 2, MXK3.27, MXK3_27, DKFZp469L2414

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 35kDa band observed in lysates of cell lines A431 and A549 (calculated MW of 38.6kDa according to NP_004030.1 and NP_001002857.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology

Restrictions: For Research Use only

Images

ABIN184687 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Takahashi, Reddy, Chirgwin et al.: "Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption." in: The Journal of biological chemistry, Vol. 269, Issue 46, pp. 28696-701, 1994 (PubMed).