Rac GTPase Activating Protein 1 (RACGAP1) antibody

Antigen: Rac GTPase Activating Protein 1 (RACGAP1)

Synonyms: ID-GAP, HsCYK-4, MgcRacGAP, racgap1, MGC69444, MGC83804, RACGAP1, MGC121578

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184690

Additional Information

Alternative name: RACGAP1 / MgcRacGAP

Gene ID: 29127

Immunogen: C-GRQGNFFASPMLK

Format: Affinity-purified

Description: All names and symbols: RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4. Official Symbol: RACGAP1. RefSeq number(s): NP_037409.1. Gene ontology terms: intracellular signaling cascade

Specificity: Human 632 aa

Application Details

Application Notes: Western blot: Approx 60kDa band seen in Human Testis lysate. Recommended for use at 0.2-1µg/ml. Please note that we observe a 60kDa band as suggested in the literature (for example, see Toure et al., [J Biol Chem 1998 Mar 13,273(11):6019-23]), which is different to the predicted MW of approx. 70kDa. Immunohistochemistry: In paraffin embedded Human Testis, shows nulear staining of many cells. Recommended concentration, 1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184690 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN184690 (1µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Reibel, Dorseuil, Stancou et al.: "A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation." in: Biochemical and biophysical research communications, Vol. 175, Issue 2, pp. 451-8, 1991 (PubMed).