Vav 2 Guanine Nucleotide Exchange Factor (VAV2) antibody

Antigen: Vav 2 Guanine Nucleotide Exchange Factor (VAV2)

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184702

Additional Information

Alternative name: VAV2

Gene ID: 7410, 22325 (mouse)

Immunogen: ETEAKYYRTLEDIEC

Format: Affinity-purified

Description: All names and symbols: VAV2, vav 2 oncogene, Oncogene VAV2. Official Symbol: VAV2. RefSeq number(s): NP_003362. Gene ontology terms: guanyl-nucleotide exchange factor activity, intracellular signaling cascade, diacylglycerol binding

Specificity: Human, Mouse 878 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 100kDa band observed in 293 lysate (predicted MW of 100kDa according to NP_003362). Recommended for use at 1-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Transcription Factors

Restrictions: For Research Use only

Images

ABIN184702 staining (1.5 ug/ml) of 293 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Henske, Short, Jozwiak et al.: "Identification of VAV2 on 9q34 and its exclusion as the tuberous sclerosis gene TSC1." in: Annals of human genetics, Vol. 59, Issue Pt 1, pp. 25-37, 1995 (PubMed).