VAV2 antibody

Catalog No. ABIN306673

This Sheep Polyclonal antibody specifically detects VAV2 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for VAV2 antibody (ABIN306673)

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This VAV2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-VAV2 Antibody

Purpose: Sheep anti Human Vav-2 DPH

Characteristics: The immunogen used for the anti-human Vav-2 DPH region was a his tagged fusion protein Vav-2 corresponding to amino acid sequence 287-578 and having a molecular weight of 100kD. Vav-2 has a molecular weight of 100 kD and shares over a 50 % homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetiic cells. Both proteins are comprised of a Dbl- homology ((DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokenesis frequently are uncoupled.

Purification: Ammonium Sulfate Precipitation

Isotype: IgG

Application Details

Application Notes: Western Blot 1:2500 -1:5000. Immunoprecipitation -Empirical Dilutions should be titrated for each application to obtain the optimal antibody titer.

Restrictions: For Research Use only

Handling

Concentration: 1mg/ml

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for VAV2

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Alternative Name: Vav-2 DPH

Background: VAV-2, Guanine nucleotide exchange factor VAV-2, Dbl homology (DH) domain

UniProt: P52735

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway