APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) antibody

Antigen: APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1)

Synonyms: APE, Apex, HAP1, Ref-1, APEX1, APEN, BAP1, MGC139790, MGC113976, zgc:66204, wu:fc13d11, hm:zehn1532, apex1, MGC76280, MGC79645, MGC78928, APX, APE1, APEX, REF1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184704

Additional Information

Alternative name: APE1 / APEX1

Gene ID: 328

Immunogen: PKRGKKGAVAEDGD-C

Format: Affinity-purified

Description: All names and symbols: APE1, APEX1, APEX, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic) endonuclease, deoxyribonuclease (apurinic or apyrimidinic). Official Symbol: APEX1. RefSeq number(s): NP_001632.2, NP_542379.1, NP_542380.1. Gene ontology terms: DNA-(apurinic or apyrimidinic site) lyase activity, uracil DNA N-glycosylase activity, endodeoxyribonuclease activity, transcription co-repressor activity, 3'-5' exonuclease activity, endonuclease activity, DNA binding, transcription co-activator activity, base-excision repair, DNA repair, transcription from Pol II promoter, obsolete, nucleus

Specificity: NP_001632.2, NP_542379.1 and NP_542380.1 are varients that represent the same protein. Human 318 aa, 318 aa, 318 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in Human epidermoid fibroblast A431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1, NP_542380.1. Recommended concentration: 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184704 staining (0.5µg/ml) of A431 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Demple, Herman, Chen: "Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 24, pp. 11450-4, 1992 (PubMed).