DKK3 (C-Term) antibody

Details for Product No. ABIN184709
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Antigen
Synonyms AW061014, C87148, REIC, RIG, Reic, p29
Epitope
C-Term
(12), (12), (9), (6), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(83), (37), (19), (13), (12), (5), (1), (1)
Host
Goat
(72), (16), (7), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(68), (53), (22), (19), (12), (10), (2), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
Options
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Availability Available
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Catalog No. ABIN184709
374.27 $
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Immunogen Peptide with sequence C-AAAALLGGEEI, from the C Terminus of the protein sequence according to NP_037385.2, NP_056965.3, NP_001018067.1.
Specificity NP_037385.2, NP_056965.3 and NP_001018067.1 are varients of the same protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DKK3 / REIC
Background Synonyms: DKK3, REIC, dickkopf homolog 3 (Xenopus laevis), DKK-3, RIG-like 5-6, RIG-like 7-1, dickkopf 3, Dickkopf gene 3, dickkopf (Xenopus laevis) homolog 3, dickkopf homolog 3, RIG, regulated in glioma
Gene ID 27122
NCBI Accession NP_001018067, NP_037385, NP_056965
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 70 kDa band observed in lysates of cell line HEK293 (calculated MW of 38.4 kDa according to NP_037385.2, NP_056965.3 and NP_001018067.1). The observed molecular weight corresponds to earlier findings in literature (Krupnik et al, Gene. 1999 Oct 1,238(2):301-13., PMID: 10570958 ). Recommended concentration: 0.03-0.1 µg/mL.
IHC: In paraffin embedded Human Brain Cortex shows vesiculate staining in cytoplasm of neuronal cell bodies. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-DKK3 (C-Term) antibody ABIN184709 staining (2µg/ml) of HEK293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
General Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).

Validation Images
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