Quinone Reductase 1 (NQO1) antibody

Antigen: Quinone Reductase 1 (NQO1)

Synonyms: DTD, QR1, DHQU, DIA4, NMOR1, NMORI

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN184714

Additional Information

Alternative name: NQO1 (Quinone reductase 1)

Gene ID: 1728, 18104 (mouse)

Immunogen: C-SIPTDNQIKARK

Format: Affinity-purified

Description: All names and symbols: NQO1, NAD(P)H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Symbol: NQO1. RefSeq number(s): NP_000894.1, NP_001020604.1, NP_001020605.1. Gene ontology terms: NAD(P)H dehydrogenase (quinone) activity, cytochrome b5 reductase activity, nitric oxide biosynthesis, response to toxin, synaptic transmission, cholinergic, xenobiotic metabolism, electron transport, cytoplasm, oxidoreductase activity

Specificity: This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1). Human, Mouse, Rat 274 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 30kDa band observed in Human Kidney and Lung lysates (calculated MW of 30.9kDa according to NP_000894.1). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Metabolism, Drugs

Restrictions: For Research Use only

Images

ABIN184714 staining (0.03µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Asher, Lotem, Kama et al.: "NQO1 stabilizes p53 through a distinct pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 3099-104, 2002 (PubMed).

van Horssen, Schreibelt, Boe et al.: "NAD(P)H:quinone oxidoreductase 1 expression in multiple sclerosis lesions." in: Free radical biology & medicine, Vol. 41, Issue 2, pp. 311-7, 2006 (PubMed).

Ritz, Wan, Diaz-Sanchez: "Sulforaphane-stimulated phase II enzyme induction inhibits cytokine production by airway epithelial cells stimulated with diesel extract." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 292, Issue 1, pp. L33-9, 2007 (PubMed).

Tanaka, Aleksunes, Cui et al.: "ANIT-induced intrahepatic cholestasis alters hepatobiliary transporter expression via Nrf2-dependent and independent signaling." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 108, Issue 2, pp. 247-57, 2009 (PubMed).

Schriner, Avanesian, Liu et al.: "Protection of human cultured cells against oxidative stress by Rhodiola rosea without activation of antioxidant defenses." in: Free radical biology & medicine, Vol. 47, Issue 5, pp. 577-84, 2009 (PubMed).