Axin antibody (C-Term)

Catalog No. ABIN184720

This Goat Polyclonal antibody specifically detects Axin in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Axin antibody (C-Term) (ABIN184720)

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Axin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Axin Antibody

Binding Specificity: C-Term

Purpose: AXIN1

Sequence: FEEKIIGKVE KVD

Specificity: This antibody is expected to recognise both reported isoforms.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-FEEKIIGKVEKVD, from the C Terminus of the protein sequence according to NP_003493.1, NP_851393.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Adrenal Gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration, 3-5 μg/mL.

Western Blot: Preliminary experiments gave bands at approx 150 kDa and 22-24 kDa in Human Brain (Hippocampus) lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe give

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: AXIN1

Background: AXIN1, axin 1, AXIN, LA16c-314G4.3, MGC52315, axis inhibition protein 1, axis inhibitor 1, fused, mouse, homolog of

Gene ID: 8312, 12005, 79257

NCBI Accession: NP_003493, NP_851393

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling