MAP/microtubule Affinity-Regulating Kinase 4 (MARK4) antibody

Antigen: MAP/microtubule Affinity-Regulating Kinase 4 (MARK4)

Synonyms: MARKL1, FLJ90097, KIAA1860, Nbla00650, C79806, Markl1, 2410090P21Rik, MARK4

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184722

Additional Information

Alternative name: MARK4

Gene ID: 57787

Immunogen: IKRKPPPGCSDSPGV

Format: Affinity-purified

Description: All names and symbols: MARK4, MAP/microtubule affinity-regulating kinase 4, MARKL1, KIAA1860, Nbla00650, MARK4 serine/threonine protein kinase, MAP/microtubule affinity-regulating kinase like 1. Official Symbol: MARK4. RefSeq number(s): NP_113605.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 70kDa band observed in human prostate lysates (calculated MW of 75.3kDa according to NP_113605.2). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Cytoskeleton

Restrictions: For Research Use only

Images

ABIN184722 (0.5µg/ml) staining of human prostate lysate (35µg protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Kato, Satoh, Okabe et al.: "Isolation of a novel human gene, MARKL1, homologous to MARK3 and its involvement in hepatocellular carcinogenesis." in: Neoplasia (New York, N.Y.), Vol. 3, Issue 1, pp. 4-9, 2001 (PubMed).