SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70) antibody

Antigen: SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70)

Synonyms: 70kDa, AV235546, HSPC321, SWAP-70, FLJ39540, KIAA0640, SWAP70

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184723

Additional Information

Alternative name: SWAP70

Gene ID: 23075

Immunogen: C-EEREKNWKEKKTTE

Format: Affinity-purified

Description: All names and symbols: SWAP70, SWAP-70 protein, SWAP-70, KIAA0640, KIAA0640 protein, FLJ39540, HSPC321. Official Symbol: SWAP70. RefSeq number(s): NP_055870.2. Gene ontology terms: calcium ion binding

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western blot: Approx 70kDa band seen in Daudi lysate. Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN184723 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Pearce, Angeli, Randolph et al.: "Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs." in: Nature immunology, Vol. 7, Issue 8, pp. 827-34, 2006 (PubMed).