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Interferon Regulatory Factor 6 (IRF6) (C-Term) antibody

Details for Product No. ABIN184730, Supplier: Log in to see
Antigen
  • IRF6
  • lps
  • pit
  • pps
  • vws
  • ofc6
  • xIRF-6
  • DKFZp469E012
  • LPS
  • OFC6
  • PIT
  • PPS
  • PPS1
  • VWS
  • VWS1
  • zgc:63500
  • AI876454
  • E230028I05Rik
  • mirf6
Epitope
C-Term
20
3
3
3
3
2
2
2
1
1
1
Reactivity
Human
80
36
27
11
6
5
5
4
4
2
1
1
1
1
1
Host
Goat
42
23
18
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
66
24
23
14
13
12
11
3
2
2
1
Supplier
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Immunogen Peptide with sequence C-TPSMQLPPALPPQ, from the C Terminus of the protein sequence according to NP_006138.1.
Blocking Peptide Blocking peptide for this product available: ABIN369981
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name IRF6 (IRF6 Antibody Abstract)
Background Synonyms: IRF6, interferon regulatory factor 6, LPS, PIT, PPS, VWS, OFC6
Gene ID 3664, 54139
NCBI Accession NP_006138
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Innate Immunity, Cytokines
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60 kDa band observed in Human Ovary lysates (calculated MW of 53.1 kDa according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6 µg/mL.
IHC: In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration: 2-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Interferon Regulatory Factor 6 (IRF6) (C-Term) antibody (ABIN184730) ABIN184730 staining (0.2µg/ml) of Human Ovary lysate (RIPA buffer, 35µg total protein...
Background publications Kondo, Schutte, Richardson et al.: "Mutations in IRF6 cause Van der Woude and popliteal pterygium syndromes. ..." in: Nature genetics, Vol. 32, Issue 2, pp. 285-9, 2002 (PubMed).