Tetraspanin 32 (TSPAN32) antibody

Antigen: Tetraspanin 32 (TSPAN32)

Synonyms: ART1, PHMX, PHEMX, TSSC6, FLJ17158, FLJ97586, MGC22455, TSPAN32

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184746

Additional Information

Alternative name: PHEMX / TSPAN32

Gene ID: 10077

Immunogen: GGLSGCPERGLSD

Format: Affinity-purified

Description: All names and symbols: TSPAN32, PHEMX, tetraspanin 32, MGC22455, PHMX, TSSC6, pan-hematopoietic expression, pan-hematopoietic expression protein, tetraspanin, tumor-suppressing STF cDNA 6, tumor-suppressing subchromosomal transferable fragment cDNA 6, tumor-suppressing subtransferable candidate 6. Official Symbol: TSPAN32. RefSeq number(s): NP_620591.1, NP_005696.1. Gene ontology terms: molecular_function unknown, biological_process unknown, cell-cell signaling, integral to membrane

Specificity: This antibody is expected to recognise human isoforms 1 and 2 (NP_620591.1, NP_005696.1) but not human isoforms 3 and 4 (NP_620592, NP_620593). Human 290 aa, 320 aa, 258 aa, 124 aa

Application Details

Application Notes: Western blot: Approx 33-35kDa band seen in HeLa lysate. Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184746 staining (2µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Lee, Brandenburg, Landes et al.: "Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting." in: Human molecular genetics, Vol. 8, Issue 4, pp. 683-90, 1999 (PubMed).