Junction Plakoglobin (JUP) antibody

Antigen: Junction Plakoglobin (JUP)

Synonyms: DP3, PDGB, PKGB, CTNNG, DPIII, ARVD12, dp3, pdgb, pkgb, ctnng, dpiii, MGC75867, MGC137835, plak

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184775

Additional Information

Alternative name: Plakoglobin / Gamma-catenin

Gene ID: 3728, 16480 (mouse)

Immunogen: C-GLRPPYPTADHMLA

Format: Affinity-purified

Description: All names and symbols: GAMMA-CATENIN, gamma-catenin, JUP, junction plakoglobin, DP3, PDGB, PKGB, DPIII. Official Symbol: JUP. RefSeq number(s): NP_002221, NP_068831. Gene ontology terms: cytoplasm, cell adhesion, soluble fraction, membrane fraction, cytoskeletal protein binding protein

Specificity: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1. Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 85kDa band observed in A431 lysate (predicted MW of 86kDa according to NP_002221, NP_068831). Recommended for use at 0.2-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184775 staining (0.2µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Knudsen, Wheelock: "Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin." in: The Journal of cell biology, Vol. 118, Issue 3, pp. 671-9, 1992 (PubMed).