JUP antibody

Catalog No. ABIN6939839

The Mouse Monoclonal anti-JUP antibody is suitable to detect JUP in samples from Human, Mouse, Rat, Chicken, Dog, Cow and Cat. It has been validated for WB, IF, IHC, FACS and ELISA.

Quick Overview for JUP antibody (ABIN6939839)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Rat, Chicken, Dog, Cow, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Clone: 15F11

Product Details anti-JUP Antibody

Purpose: Catenin, gamma (Cardiomyocyte Marker) Antibody

Specificity: It recognizes a protein of 80-87 kDa, identified as gamma-catenin. The catenins (°™, æ, Á and 79oΩL) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. °™ T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. °™ T-catenin binds to °™ E-catenin as well as to æ-catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for °™-T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant, full length chicken Catenin gamma protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HeLa, SK-BR3, A431, HT20, 293, HepG2, A549 or MCF-7 cells. Skin.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: JUP

Background:

ARVD12, Catenin (cadherin-associated protein), gamma 80 kDa, Catenin gamma, CTNNG, Desmoplakin III, Desmoplakin-3, DP3, DPIII, Junction Plakoglobin, PDGB, PKGB

HeLa, SK-BR3 or MCF-7 cells.

Molecular Weight: 80-87kDa

Gene ID: 3728, 514174

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location