Carbonyl Reductase 1 (CBR1) antibody

Antigen: Carbonyl Reductase 1 (CBR1)

Synonyms: CBR, hCBR1, SDR21C1, CR, Cbr, AW261796, MGC124927, CBR1, 9-KPR, MGC128388, DKFZp469D1211, LOC100222525, MGC131152, PSCD

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184784

Additional Information

Alternative name: CBR1

Gene ID: 873

Immunogen: Peptide with sequence C-HGQFVSEKRVEQW, from the C Terminus of the protein sequence according to NP_001748.1

Format: Affinity-purified

Description: All names and symbols: CBR1, carbonyl reductase 1, CBR, carbonyl reductase (NADPH), carbonyl reductase (NADPH) 1, hCBR1, prostaglandin 9-ketoreductase, prostaglandin-E(2) 9-reductase, SDR21C1, short chain dehydrogenase/reductase family 21C, member 1. Official Symbol: CBR1. RefSeq number(s): NP_001748.1. Gene ontology terms: carbonyl reductase (NADPH) activity, metabolism, cytosol, oxidoreductase activity.

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 30-35kDa band seen in Human Liver and Kidney lysates [Calculated MW of 30.4kDa according to NP_001748.1]. Recommended for use at 0.2-0.5µg/ml. Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration, 1-2µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Research Area: Metabolism

Restrictions: For Research Use only

Publications

Publications: Balcz, Kirchner, Cairns et al.: "Increased brain protein levels of carbonyl reductase and alcohol dehydrogenase in Down syndrome and Alzheimer's disease." in: Journal of neural transmission. Supplementum, Issue 61, pp. 193-201, 2001 (PubMed).