Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1) antibody

Antigen: Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1)

Synonyms: ALR, ARM, DD3, ALDR1, MGC1380, MGC12529, Akr1a1, 2610201A18Rik, Akr1a4, ALR1, MGC81878, akr1a1, zgc:100940, AKR1A1, MGC110225, zgc:110225, DKFZp469F2031

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184795

Additional Information

Alternative name: Aldehyde Reductase

Gene ID: 10327, 58810 (mouse), 78959 (rat)

Immunogen: Peptide with sequence C-DAGHPLYPFNDPY, from the C Terminus of the protein sequence according to NP_006057.1, NP_697021.1

Format: Affinity-purified

Description: All names and symbols: aldehyde reductase, AKR1A1, aldo-keto reductase family 1, member A1 (aldehyde reductase), ALDR1, ALR, MGC12529, MGC1380, alcohol dehydrogenase, aldo-keto reductase family 1, member A1, DD3, OTTHUMP00000009240, dihydrodiol dehydrogenase 3. Official Symbol: AKR1A1. RefSeq number(s): NP_006057.1, NP_697021.1. Gene ontology terms: glucose metabolism, aldehyde reductase, aldehyde metabolism, electron transporter Both reported variants (NP_006057.1and NP_697021.1) represent identical protein.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 37-40kDa band observed in Human Placenta and Liver lysates (predicted MW of 37kDa according to NP_006057). Recommended for use at 0.5-2µg/ml. Full figure caption: A) HEK293 overexpressing AKR1A1 (RC200302) and probed with EB05538 (mock transfection in first lane), tested by Origene. B) EB05538 staining (1µg/ml) of Human Placenta lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Immunohistochemistry: In paraffin embedded Human Kidney shows Brush Border staining in PCT. Recommended concentration, 2-3µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Fujii, Hamaoka, Matsumoto et al.: "The structural organization of the human aldehyde reductase gene, AKR1A1, and mapping to chromosome 1p33-->p32." in: Cytogenetics and cell genetics, Vol. 84, Issue 3-4, pp. 230-2, 1999 (PubMed).