Interferon Regulatory Factor 5 (IRF5) antibody

Antigen: Interferon Regulatory Factor 5 (IRF5)

Synonyms: SLEB10, mirf5, AW491843, MGC76986, zgc:76986, im:7155364, IRF5, irf5

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN184812

Additional Information

Alternative name: IRF5

Gene ID: 3663

Immunogen: C-QGPWPMHPAGMQ

Description: All names and symbols: IRF5, interferon regulatory factor 5, SLEB10. Official Symbol: IRF5. RefSeq number(s): NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1. Gene ontology terms: RNA polymerase III transcription factor activity, transcription factor activity, cell growth and/or maintenance, regulation of transcription, DNA-dependent, nucleus

Specificity: This antibody is expected to recognize all four reported isoforms (NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1). Reported variants NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein: Reported variants NP_116032.1, NP_001092100.1 represent identical protein: Human, Mouse, Rat, Cow 504 aa, 498 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 57kDa band observed in lysates of cell line A549. In transfected HEK293 transiently expressing IRF5 a strong band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Innate Immunity, Cytokines

Restrictions: For Research Use only

Images

ABIN184812 (0.1µg/ml) staining of lysate of cell line A549 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing human IRF5 and probed with ABIN184812 at 1ug/ml (mock transfection in lane B).

Publications

Barnes, Moore, Pitha: "Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23382-90, 2001 (PubMed).