Ring Finger Protein 31 (RNF31) (C-Term) antibody

Antigen: Ring Finger Protein 31 (RNF31)

Synonyms: ZIBRA, FLJ10111, FLJ23501, MGC19975, RNF31

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184814

Additional Information

Alternative name: RNF31 / ZIBRA (C terminus)

Gene ID: 55072

Immunogen: C-EEVPLGQSIPRRRK

Format: Affinity-purified

Description: All names and symbols: ZIBRA, RNF31, FLJ10111, FLJ23501, zinc in-between-ring-finger ubiquitin-associated domain. Official Symbol: RNF31. RefSeq number(s): AAH12077.1

Specificity: Human 1072 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 120kDa band observed in lysates of cell line HepG2 (calculated MW of 120kDa according to AAH12077.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184814 staining (2µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: van der Reijden, Erpelinck-Verschueren, Loewenberg et al.: "TRIADs: a new class of proteins with a novel cysteine-rich signature." in: Protein science : a publication of the Protein Society, Vol. 8, Issue 7, pp. 1557-61, 1999 (PubMed).