RNF31 antibody (Middle Region)

Catalog No. ABIN2774753

Cited in 8+ publications. The Rabbit Polyclonal anti-RNF31 antibody (ABIN2774753) specifically detects RNF31 in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Horse and Cow samples.

Quick Overview for RNF31 antibody (Middle Region) (ABIN2774753)

Target: RNF31 (Ring Finger Protein 31 (RNF31))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF31 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF31 Antibody

Binding Specificity: Middle Region

Purpose: RNF31 Antibody - middle region

Sequence: SLINAHSLDP ATLYEVEELE TATERYLHVR PQPLAGEDPP AYQARLLQKL

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RNF31. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF31

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43241-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RNF31 antibody (ABIN2774753)

Shibata, Tokunaga, Goto, Komatsu, Gohda, Saeki, Tanaka, Takahashi, Sawasaki, Inoue, Oshiumi, Seya, Nakano, Tanaka, Iwai, Inoue: "HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains." in: PLoS pathogens, Vol. 13, Issue 1, pp. e1006162, (2017) (PubMed).

Lafont, Kantari-Mimoun, Draber, De Miguel, Hartwig, Reichert, Kupka, Shimizu, Taraborrelli, Spit, Sprick, Walczak: "The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death." in: The EMBO journal, Vol. 36, Issue 9, pp. 1147-1166, (2017) (PubMed).

Kupka, De Miguel, Draber, Martino, Surinova, Rittinger, Walczak: "SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes." in: Cell reports, Vol. 16, Issue 9, pp. 2271-80, (2016) (PubMed).

Queisser, Dada, Deiss-Yehiely, Angulo, Zhou, Kouri, Knab, Liu, Stegh, DeCamp, Budinger, Chandel, Ciechanover, Iwai, Sznajder: "HOIL-1L functions as the PKCζ ubiquitin ligase to promote lung tumor growth." in: American journal of respiratory and critical care medicine, Vol. 190, Issue 6, pp. 688-98, (2014) (PubMed).

Blackwell, Zhang, Workman, Ting, Iwai, Habelhah: "Two coordinated mechanisms underlie tumor necrosis factor alpha-induced immediate and delayed I?B kinase activation." in: Molecular and cellular biology, Vol. 33, Issue 10, pp. 1901-15, (2013) (PubMed).

Liu, Chen, Cai, Wu, Chen, Wu, Sun, Chen: "MAVS recruits multiple ubiquitin E3 ligases to activate antiviral signaling cascades." in: eLife, Vol. 2, pp. e00785, (2013) (PubMed).

Smit, Monteferrario, Noordermeer, van Dijk, van der Reijden, Sixma: "The E3 ligase HOIP specifies linear ubiquitin chain assembly through its RING-IBR-RING domain and the unique LDD extension." in: The EMBO journal, Vol. 31, Issue 19, pp. 3833-44, (2012) (PubMed).

Sieber, Lange, Kollmorgen, Erhardt, Quaas, Gontarewicz, Sass, Tiegs, Kreienkamp: "Sharpin contributes to TNF? dependent NF?B activation and anti-apoptotic signalling in hepatocytes." in: PLoS ONE, Vol. 7, Issue 1, pp. e29993, (2012) (PubMed).

Target Details for RNF31

Target: RNF31 (Ring Finger Protein 31 (RNF31))

Alternative Name: RNF31

Background:

Background Information: PWP2 contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.

Gene Name: Ring finger protein 31

Alternative Symbols: HOIP, Paul, ZIBRA

Protein Name: E3 ubiquitin-protein ligase RNF31

Molecular Weight: 120kDa

Gene ID: 55072

NCBI Accession: NP_060469

UniProt: Q96EP0