Forkhead Box G1 (FOXG1) antibody

Antigen: Forkhead Box G1 (FOXG1)

Synonyms: BF1, BF2, QIN, FKH2, HBF2, HFK1, HFK2, HFK3, KHL2, FHKL3, FKHL1, FKHL2, FKHL3, FKHL4, HBF-1, HBF-2, HBF-3, FOXG1A, FOXG1B, FOXG1C, HBF-G2, Bf1, BF-1, Hfh9, Hfhbf1, 2900064B05Rik, BF1A, Fkhl1, RATBF1A, bf1, zgc:109850, foxg1, XBF-1, foxg1b-a, FOXG1, CBF-1, qin, XBf1, fkh2, hfk1, fkhl1, fkhl4, hbf-1, foxg1b, bf-1, BmFOXG1, MGC85969, zgc:85969

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184820

Additional Information

Alternative name: FOXG1A (BF2) and FOXG1B (BF1)

Gene ID: 2291, 23, 15228 (mouse)

Immunogen: C-HQNQGSSSNPLIH

Format: Affinity-purified

Description: All names and symbols: BF1, BF2, forkhead box G1B, FKH2, FKHL1, FKHL4, HBF-1, HFK1, QIN, forkhead (Drosophila)-like 1, forkhead (Drosophila)-like 4, oncogene QIN, forkhead box G1A, FKHL2, HBF-2, HBF-G2, HBF2, HFK2, KHL2, Forkhead, drosophila, homolog-like 2, brain factor 2, forkhead (Drosophila)-like 2. Official Symbol: FOXG1A / FOXG1B. RefSeq number(s): CAA55038.1 (FOXG1A), NP_005240.3 (FOXG1B). Gene ontology terms: transcription factor activity, regulation of transcription, DNA-dependent, nucleus

Specificity: This antibody will recognise both human FOXG1A and FOXG1B, which are almost completely identical. Human, Mouse, Rat 469 aa

Synonyms: BF1, BF2, QIN, FKH2, HBF2, HFK1, HFK2, HFK3, KHL2, FHKL3, FKHL1, FKHL2, FKHL3, FKHL4, HBF-1, HBF-2, HBF-3, FOXG1A, FOXG1B, FOXG1C, HBF-G2, Bf1, BF-1, Hfh9, Hfhbf1, 2900064B05Rik, BF1A, Fkhl1, RATBF1A, bf1, zgc:109850, foxg1, XBF-1, foxg1b-a, FOXG1, CBF-1, qin, XBf1, fkh2, hfk1, fkhl1, fkhl4, hbf-1, foxg1b, bf-1, BmFOXG1, MGC85969, zgc:85969

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 48 kDa band observed in Human Brain lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B) and 50.5kDa according to CAA55038.1 (FOXG1A). Recommended concentration: 0.3-1.0µg/ml. An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184820 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Wiese, Murphy, Schlung et al.: "The genes for human brain factor 1 and 2, members of the fork head gene family, are clustered on chromosome 14q." in: Biochimica et biophysica acta, Vol. 1262, Issue 2-3, pp. 105-12, 1995 (PubMed).