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Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) antibody

Antigen

Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1)

Synonyms S6K, PS6K, S6K1, STK14A, p70-S6K, p70-alpha, p70(S6K)-alpha
Clonality Polyclonal
Host
Reactivity
Alternatives

Human

Application
Alternatives ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN184828
Quantity 100 ug  (Variants)
Price 501.80 $   Plus shipping costs $35.00
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Additional Information

Alternative name p70S6K / RPS6KB1
Gene ID 6198, 72508 (mouse), 83840 (rat)
Immunogen C-MISKRPEHLRMNL
Format Affinity-purified
Description All names and symbols: RPS6KB1, p70-S6K, ribosomal protein S6 kinase, 70kD, polypeptide 1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-alpha, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, ribosomal protein S6 kinase, 70kD, polypeptide 1, serine/threonine kinase 14 alpha. Official Symbol: RPS6KB1. RefSeq number(s): NP_003152.1. Gene ontology terms: protein kinase, signal transduction
Specificity Human, Mouse, Rat, Rabbit

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution >1:128,000. Western Blot: Approx 65-70kDa band observed in Human Skeletal Muscle and Placenta lysates (predicted MW of 60kDa according to NP_003152.1). Recommended for use at 0.2-1µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Publications Grove, Banerjee, Balasubramanyam et al.: "Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini." in: Molecular and cellular biology, Vol. 11, Issue 11, pp. 5541-50, 1991 (PubMed).

Alternatives

Alternatives for antigen "Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1)", type "Antibodies"
Hosts Rabbit (172), Mouse (15), Goat (5)
Reactivities Human (187), Mouse (Murine) (117), Rat (Rattus) (108), Cow (Bovine) (25), Dog (Canine) (20), Pig (Porcine) (18), Chicken (16), Monkey (2), Rabbit (2), Sheep (Ovine) (2), Cat (Feline) (1), Horse (Equine) (1), Xenopus laevis (1)
Applications Western Blotting (WB) (136), Immunofluorescence (IF) (84), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (55), Immunohistochemistry (IHC) (54), Flow Cytometry (FACS) (36), Enzyme Immunoassay (EIA) (34), ELISA (27), Immunoprecipitation (IP) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Dot Blot (Dot) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2)
Conjugates Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Flour 488 (1)
Epitopes pSer424 (11), pSer411 (10), pThr421 (9), pThr421/pSer424 (4), C-Term (3), Center (2), N-Term (2), pSer418 (2), pSer434 (2), pThr389 (2), S404 (1), S411 (1), S418 (1), S424 (1), T421 (1), pS418 (1), pS424 (1), pSer447 (1), pThr444 (1)