ACSL4 antibody (C-Term)

Catalog No. ABIN184846

This Goat Polyclonal antibody specifically detects ACSL4 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for ACSL4 antibody (C-Term) (ABIN184846)

Target: ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ACSL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ACSL4 Antibody

Binding Specificity: C-Term

Purpose: FACL4 / ACSL4

Sequence: HYLKDIERMY GGK

Specificity: This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HYLKDIERMYGGK, from the C Terminus of the protein sequence according to NP_004449.1, NP_075266.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 80-85 kDa band observed in lysates of cell line HepG2 and approx 75 kDa in preliminary testing of Human Adipose lysate (calculated MW of 79.2 kDa according to NP_075266.1 and 74.4 kDa according to NP_004449.1). Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Golgi and Endoplasmic reticulum of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACSL4

Target: ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

Alternative Name: ACSL4

Background: ACSL4, acyl-CoA synthetase long-chain family member 4, FACL4, fatty-acid-Coenzyme A ligase, long-chain 4, ACS4, LACS 4, lignoceroyl-CoA synthase, Fatty acid coenzyme A ligase, long-chain 4, MRX63, LACS4, MRX68, acyl-CoA synthetase 4, long-chain fatty-acid

Gene ID: 2182, 50790, 113976

NCBI Accession: NP_004449, NP_075266