Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) antibody

Antigen: Enhancer of Zeste Homolog 1 (Drosophila) (EZH1)

Synonyms: KIAA0388, MGC136829, zgc:136829, AW557915, mKIAA0388, EZH1, MGC157156

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184879

Additional Information

Alternative name: EZH1

Gene ID: 2145, 14055 (mouse)

Immunogen: EIPNPPTSKCITY

Description: All names and symbols: EZH1, enhancer of zeste homolog 1 (Drosophila), KIAA0388. Official Symbol: EZH1. RefSeq number(s): NP_001982.2. Gene ontology terms: chromatin binding, regulation of transcription, DNA-dependent, embryogenesis and morphogenesis, nucleus

Specificity: Human, Mouse 747 aa

Application Details

Application Notes: Western blot: Approx 75-80kDa band seen in MOLT-4 lysate [Predicted MW of 82kDa according to NP_001982]. Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling

Restrictions: For Research Use only

Images

ABIN184879 staining (2µg/ml) of MOLT-4 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Abel, Brody, Valdes et al.: "Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1." in: Genomics, Vol. 37, Issue 2, pp. 161-71, 1997 (PubMed).