Tripartite Motif Containing 54 (TRIM54) antibody

Antigen: Tripartite Motif Containing 54 (TRIM54)

Synonyms: MURF, RNF30, MURF-3, Rnf30, MGC80214, MGC137273, si:dkey-221h15.2, DKFZp468L1522

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184954

Additional Information

Alternative name: MURF3 / TRIM54

Gene ID: 57159

Immunogen: C-GSAGPEEERPDGP

Format: Affinity-purified

Description: All names and symbols: TRIM54, MURF-3, tripartite motif-containing 54, RNF30, MURF, ring finger protein 30, muscle-specific RING-finger protein 3, muscle-specific RING-finger protein homolog. Official Symbol: TRIM54. RefSeq number(s): NP_115935

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: experiments gave bands at approx 45kDa and 38kDa in human skeletal muscle lysate after 1µg/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184954 staining (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Centner, Yano, Kimura et al.: "Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain." in: Journal of molecular biology, Vol. 306, Issue 4, pp. 717-26, 2001 (PubMed).